Hotspot

rpoB-trnC-GCA

LSC · 25786-27883 · 2098 bp

Back to Beccarinda

Pi 0.0208

MarkerSeek score 56.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 2098
variable_sites: 43
pi: 0.0208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GACCCATCCTCGCATGAATT	ATCCTAGATGCGAACGAGGA	50.0	50.0	58.0	58.0	2765-2769 bp	1.0	82.0
rpoB-trnC-GCA_p2	GACCCATCCTCGCATGAATT	TCCTAGATGCGAACGAGGAT	50.0	50.0	58.0	58.0	2764-2768 bp	1.0	82.0
rpoB-trnC-GCA_p3	GACCCATCCTCGCATGAATT	TGTTGTTTGATCAGGCGACA	50.0	45.0	58.0	58.0	2596-2600 bp	1.0	82.0
rpoB-trnC-GCA_p4	GACGATATCGAGCTGGAACC	ATCCTAGATGCGAACGAGGA	55.0	50.0	57.9	58.0	2936-2940 bp	1.0	81.7
rpoB-trnC-GCA_p5	GACGATATCGAGCTGGAACC	TCCTAGATGCGAACGAGGAT	55.0	50.0	57.9	58.0	2935-2939 bp	1.0	81.7