Hotspot

trnS-GCU-trnS-CGA

LSC · 8140-8888 · 749 bp

Back to Beccarinda

Pi 0.0275
MarkerSeek score 62.20
Primer available yes
Label trnS-GCU-trnS-CGA

Feature Metadata

label_name
trnS-GCU-trnS-CGA
feature_type
igs
region
LSC
length_bp
749
variable_sites
20
pi
0.0275
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnS-CGA_p1 CAATCCGACGCTTTAGTCCA ACCACTAAACTATACCCGCT 50.0 45.0 58.0 55.6 804 bp 0.5 41.1
trnS-GCU-trnS-CGA_p2 AATCCGACGCTTTAGTCCAC ACCACTAAACTATACCCGCT 50.0 45.0 58.0 55.6 803 bp 0.5 41.1
trnS-GCU-trnS-CGA_p3 ACGGAAAGAGAGGGATTCGA ACCACTAAACTATACCCGCT 50.0 45.0 58.2 55.6 858 bp 0.5 40.0
trnS-GCU-trnS-CGA_p4 AGCAATCCGACGCTTTAGTC ACCACTAAACTATACCCGCT 50.0 45.0 58.4 55.6 806 bp 0.5 38.3
trnS-GCU-trnS-CGA_p5 CGACGCTTTAGTCCACTCAG ACCACTAAACTATACCCGCT 55.0 45.0 58.4 55.6 799 bp 0.5 38.1