Hotspot

trnH-GUG-psbA

LSC · 87-386 · 300 bp

Back to Beccarinda

Pi 0.115

MarkerSeek score 86.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 300
variable_sites: 33
pi: 0.115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 86.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	362-366 bp	1.0	88.7
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	376-380 bp	1.0	88.3
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	375-379 bp	1.0	88.3
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	377-381 bp	1.0	88.2
trnH-GUG-psbA_p5	TTTAGTCATGGGCGAACGAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.3	57.9	429 bp	0.5	57.1