Hotspot

clpP

LSC · 71953-74134 · 2182 bp

Back to Bauhinia

Pi 0.0386

MarkerSeek score 68.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2182
variable_sites: 293
pi: 0.0386
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CTGAACGAGTCGCACATACA	TACGCTTCCGCATCAAAGAA	50.0	45.0	58.0	57.9	2533-2571 bp	0.875	80.2
clpP_p2	CTGAACGAGTCGCACATACA	TTACGCTTCCGCATCAAAGA	50.0	45.0	58.0	57.9	2534-2572 bp	0.875	80.2
clpP_p3	TGACCCTGTTAGTCCGTTCT	TACGCTTCCGCATCAAAGAA	50.0	45.0	58.0	57.9	2863-2901 bp	0.875	80.0
clpP_p4	TGACCCTGTTAGTCCGTTCT	TTACGCTTCCGCATCAAAGA	50.0	45.0	58.0	57.9	2864-2902 bp	0.875	80.0
clpP_p5	TGACCCTGTTAGTCCGTTCT	CATTGTTACGCTTCCGCATC	50.0	50.0	58.0	57.9	2869-2907 bp	0.875	79.9