Hotspot

accD

LSC · 59317-60837 · 1521 bp

Back to Bauhinia

Pi 0.0413

MarkerSeek score 69.30

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1521
variable_sites: 234
pi: 0.0413
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCTTTTCATGCCGTGTTGGA	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	2508-2792 bp	0.875	81.5
accD_p2	TCTTTTCATGCCGTGTTGGA	AAGGAAGCCATTGCAATTGC	45.0	45.0	58.0	57.9	2515-2799 bp	0.875	80.9
accD_p3	TCTTTTCATGCCGTGTTGGA	TCTTGCCTAATTTCGCGGAA	45.0	45.0	58.0	57.9	2162-2230 bp	0.75	72.9
accD_p4	TCTTTTCATGCCGTGTTGGA	TGCCTAATTTCGCGGAAGAA	45.0	45.0	58.0	57.9	2159-2227 bp	0.75	72.9
accD_p5	TCTTTTCATGCCGTGTTGGA	TTGCCTAATTTCGCGGAAGA	45.0	45.0	58.0	57.9	2160-2228 bp	0.75	72.9