Hotspot

ndhG-ndhI

SSC · 120760-121167 · 408 bp

Back to Bauhinia

Pi 0.0521

MarkerSeek score 72.10

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 408
variable_sites: 68
pi: 0.0521
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GTGCTGCAGCTACAAAATGG	AATCAAATTGCTTTGGGCCG	50.0	45.0	58.0	57.9	675-766 bp	0.875	86.1
ndhG-ndhI_p2	GATTTCTTACTGGCGAGCCA	AATCAAATTGCTTTGGGCCG	50.0	45.0	58.0	57.9	1028-1119 bp	0.875	83.2
ndhG-ndhI_p3	GTGCTGCAGCTACAAAATGG	TGCCTAGGCTACGGATAGAC	50.0	55.0	58.0	58.1	1277-1356 bp	0.875	78.7
ndhG-ndhI_p4	AAGACCCGATCCCAGAAAGA	TGCCTAGGCTACGGATAGAC	50.0	55.0	58.0	58.1	1150-1229 bp	0.875	78.7
ndhG-ndhI_p5	GATTTCTTACTGGCGAGCCA	TGCCTAGGCTACGGATAGAC	50.0	55.0	58.0	58.1	1630-1709 bp	0.75	71.8