Hotspot

ndhF-rpl32

SSC · 114639-115659 · 1021 bp

Back to Bauhinia

Pi 0.0408

MarkerSeek score 72.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1021
variable_sites: 126
pi: 0.0408
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TAGGGCGTGTGAACCAAAAA	TTCCATGCTGCCCAATATCC	45.0	50.0	57.9	58.0	1618-1636 bp	0.625	73.6
ndhF-rpl32_p2	TAGGGCGTGTGAACCAAAAA	CATGCTGCCCAATATCCCTT	45.0	50.0	57.9	58.0	1615-1633 bp	0.625	73.5
ndhF-rpl32_p3	GAAACTGCCCGTTATCCGAT	TTCCATGCTGCCCAATATCC	50.0	50.0	58.1	58.0	1719 bp	0.25	49.6
ndhF-rpl32_p4	GAAACTGCCCGTTATCCGAT	CATGCTGCCCAATATCCCTT	50.0	50.0	58.1	58.0	1716 bp	0.25	49.6
ndhF-rpl32_p5	GAAACTGCCCGTTATCCGAT	GGTGGAGACCTTGTTGTGTT	50.0	50.0	58.1	57.9	1932 bp	0.25	49.2