Hotspot

rps3-rps19

LSC · 85816-86383 · 568 bp

Back to Bauhinia

Pi 0.0625

MarkerSeek score 74.60

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 568
variable_sites: 128
pi: 0.0625
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	AGTCTCAAAACGAGCGGATC	TAGTCACTTGGTCCAGAGCA	50.0	50.0	58.0	58.0	777-792 bp	0.875	80.5
rps3-rps19_p2	GTCTCAAAACGAGCGGATCT	TAGTCACTTGGTCCAGAGCA	50.0	50.0	58.0	58.0	776-791 bp	0.875	80.5
rps3-rps19_p3	AGTCTCAAAACGAGCGGATC	AACCCTGTAGACCATCCACA	50.0	50.0	58.0	58.0	1118-1133 bp	0.875	79.2
rps3-rps19_p4	GTCTCAAAACGAGCGGATCT	AACCCTGTAGACCATCCACA	50.0	50.0	58.0	58.0	1117-1132 bp	0.875	79.2
rps3-rps19_p5	GTCTCAAAACGAGCGGATCT	CCGGATCGAAATGTTGGCTA	50.0	50.0	58.0	58.1	1170-1185 bp	0.875	78.9