Hotspot

rbcL-accD

LSC · 58491-59316 · 826 bp

Back to Bauhinia

Pi 0.0425

MarkerSeek score 69.70

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 826
variable_sites: 118
pi: 0.0425
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AGTCCTGAATTAGCTGCTGC	CTCCCTGTTAGACAACGTCG	50.0	55.0	58.0	58.0	819-949 bp	0.875	81.4
rbcL-accD_p2	ATGAAATTATCCGTGCGGCT	CTCCCTGTTAGACAACGTCG	45.0	55.0	58.0	58.0	848-978 bp	0.875	81.3
rbcL-accD_p3	ATGAAATTATCCGTGCGGCT	GAAATTCCGAATGTCCCCGA	45.0	50.0	58.0	58.0	1025-1155 bp	0.875	81.1
rbcL-accD_p4	AGTCCTGAATTAGCTGCTGC	TCTTCGCATTGAACCCACAA	50.0	45.0	58.0	58.0	1466-1575 bp	0.875	80.8
rbcL-accD_p5	ATGAAATTATCCGTGCGGCT	TCTTCGCATTGAACCCACAA	45.0	45.0	58.0	58.0	1495-1604 bp	0.875	80.7