Hotspot

ndhC-trnV-UAC

LSC · 52647-53207 · 561 bp

Back to Bauhinia

Pi 0.0317

MarkerSeek score 67.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 561
variable_sites: 52
pi: 0.0317
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ACTCGATATTATTAGAAATGCCCA	GTTCGAGTCCGTATAGCCCT	33.3	55.0	55.8	58.7	519-651 bp	1.0	50.9
ndhC-trnV-UAC_p2	ACTCGATATTATTAGAAATGCCCA	AGAAGGTCTACGGTTCGAGT	33.3	50.0	55.8	57.8	531-663 bp	1.0	50.8
ndhC-trnV-UAC_p3	ACTCGATATTATTAGAAATGCCCAA	AGAAGGTCTACGGTTCGAGT	32.0	50.0	56.5	57.8	531-663 bp	1.0	50.8
ndhC-trnV-UAC_p4	ACTCGATATTATTAGAAATGCCCA	GAAGGTCTACGGTTCGAGTC	33.3	55.0	55.8	57.5	530-662 bp	1.0	50.8
ndhC-trnV-UAC_p5	ACTCGATATTATTAGAAATGCCCA	AAGGTCTACGGTTCGAGTCC	33.3	55.0	55.8	58.8	529-661 bp	1.0	50.8