Hotspot

psbZ-trnG-GCC

LSC · 37896-38367 · 472 bp

Back to Bauhinia

Pi 0.0493

MarkerSeek score 72.10

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 472
variable_sites: 78
pi: 0.0493
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CAACCACTCAGCCATCTCTC	CTTGAGGTCACGGGTTCAAA	55.0	50.0	58.0	58.0	1299-1401 bp	1.0	90.7
psbZ-trnG-GCC_p2	CAACCACTCAGCCATCTCTC	CTTGTGTTTATCGCGGGGTA	55.0	50.0	58.0	58.0	1350-1408 bp	0.875	81.9
psbZ-trnG-GCC_p3	CAACCACTCAGCCATCTCTC	TTGTGTTTATCGCGGGGTAG	55.0	50.0	58.0	58.0	1349-1407 bp	0.875	81.9
psbZ-trnG-GCC_p4	CAACCACTCAGCCATCTCTC	CGATCATAGGAGGGTCCCTT	55.0	55.0	58.0	58.0	1458-1516 bp	0.875	81.8
psbZ-trnG-GCC_p5	CAACCACTCAGCCATCTCTC	CGTTGTTTTTCGACCGGAAG	55.0	50.0	58.0	58.0	1622-1680 bp	0.875	81.5