Hotspot

rpl32-trnL-UAG

SSC · 112033-112807 · 775 bp

Back to Bassia

Pi 0.0237
MarkerSeek score 64.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
775
variable_sites
37
pi
0.0237
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCAGCGTTAAAAGCCTTTT CTCGCACTGCTTCCTAAGAG 45.0 55.0 57.9 58.1 898-918 bp 1.0 83.7
rpl32-trnL-UAG_p2 GGCAGCGTTAAAAGCCTTTT ATCCGCATAAAAGAGCTGCA 45.0 45.0 57.9 58.0 1531-1556 bp 1.0 82.7
rpl32-trnL-UAG_p3 GGCAGCGTTAAAAGCCTTTT TCCGCATAAAAGAGCTGCAT 45.0 45.0 57.9 58.0 1530-1555 bp 1.0 82.7
rpl32-trnL-UAG_p4 GGCAGCGTTAAAAGCCTTTT TCCGACGTAGCAAATCCTTG 45.0 50.0 57.9 58.0 1418-1426 bp 0.8 74.4
rpl32-trnL-UAG_p5 GGCAGCGTTAAAAGCCTTTT GTCCGACGTAGCAAATCCTT 45.0 50.0 57.9 58.0 1419-1427 bp 0.8 74.4