Hotspot

petA-psbJ

LSC · 62775-63745 · 971 bp

Back to Bassia

Pi 0.0221
MarkerSeek score 66.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
971
variable_sites
46
pi
0.0221
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CGAGAAGGTTCAACTGTCCG CTGGATTAGGCTCATCACTGT 55.0 47.6 58.6 57.2 1026-1036 bp 1.0 65.0
petA-psbJ_p2 GAGAAGGTTCAACTGTCCGA CTGGATTAGGCTCATCACTGT 50.0 47.6 57.2 57.2 1025-1035 bp 1.0 63.2
petA-psbJ_p3 TCGAGAAGGTTCAACTGTCC CTGGATTAGGCTCATCACTGT 50.0 47.6 57.2 57.2 1027-1037 bp 1.0 63.2
petA-psbJ_p4 CGAGAAGGTTCAACTGTCCG TGGATTAGGCTCATCACTGT 55.0 45.0 58.6 55.9 1025-1035 bp 1.0 63.1
petA-psbJ_p5 CGAGAAGGTTCAACTGTCCG TCTGGATTAGGCTCATCACTG 55.0 47.6 58.6 56.9 1027-1037 bp 1.0 62.8