Hotspot

trnM-CAU-atpE

LSC · 52355-52655 · 301 bp

Back to Bassia

Pi 0.0481
MarkerSeek score 76.20
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
301
variable_sites
31
pi
0.0481
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 GGCGAGAGTCATTGGTTCAA CACGAGTGGAAGCTAGCAAT 50.0 50.0 58.2 58.0 352-368 bp 1.0 89.2
trnM-CAU-atpE_p2 GGCGAGAGTCATTGGTTCAA GACGAGCTAGAACACGAGTG 50.0 55.0 58.2 58.1 364-380 bp 1.0 88.2
trnM-CAU-atpE_p3 ATTGCTTTCATACGGCGAGA CACGAGTGGAAGCTAGCAAT 45.0 50.0 57.7 58.0 365-381 bp 1.0 88.0
trnM-CAU-atpE_p4 GGCGAGAGTCATTGGTTCAA CAGACGAGCTAGAACACGAG 50.0 55.0 58.2 57.8 366-382 bp 1.0 87.8
trnM-CAU-atpE_p5 GGCGAGAGTCATTGGTTCAA CTCAGACGAGCTAGAACACG 50.0 55.0 58.2 57.8 368-384 bp 1.0 87.7