Hotspot

trnD-GUC-trnY-GUA

LSC · 30268-30638 · 371 bp

Back to Bassia

Pi 0.0327
MarkerSeek score 71.70
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
371
variable_sites
26
pi
0.0327
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GCGGTGCTCTAACCAATTGA TGGCAATATGTCTACGCTGG 50.0 50.0 58.6 57.8 434-457 bp 1.0 81.0
trnD-GUC-trnY-GUA_p2 GCGGTGCTCTAACCAATTGA GGCAATATGTCTACGCTGGT 50.0 50.0 58.6 57.8 433-456 bp 1.0 81.0
trnD-GUC-trnY-GUA_p3 GCGGTGCTCTAACCAATTGA GGGGACGGACTGTAAATTCG 50.0 55.0 58.6 58.4 455-478 bp 1.0 79.8
trnD-GUC-trnY-GUA_p4 GCGGTGCTCTAACCAATTGA GGGACGGACTGTAAATTCGT 50.0 50.0 58.6 57.6 454-477 bp 1.0 79.6
trnD-GUC-trnY-GUA_p5 GCGGTGCTCTAACCAATTGA GATTCTTCCTGGGTCGATGC 50.0 55.0 58.6 58.4 488-511 bp 1.0 78.8