Hotspot

trnR-UCU-atpA

LSC · 9871-9960 · 90 bp

Back to Bassia

Pi 0.0881
MarkerSeek score 72.50
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
90
variable_sites
14
pi
0.0881
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 ATCGGAATGAAAAGCGTCCA TGGAACGCTTTCTACTTCAAGA 45.0 40.9 57.9 57.7 201-208 bp 1.0 69.5
trnR-UCU-atpA_p2 TCGGAATGAAAAGCGTCCAT TGGAACGCTTTCTACTTCAAGA 45.0 40.9 57.9 57.7 200-207 bp 1.0 69.5
trnR-UCU-atpA_p3 ATCGGAATGAAAAGCGTCCA TGGAACGCTTTCTACTTCAAG 45.0 42.9 57.9 56.2 201-208 bp 1.0 65.8
trnR-UCU-atpA_p4 TCGGAATGAAAAGCGTCCAT TGGAACGCTTTCTACTTCAAG 45.0 42.9 57.9 56.2 200-207 bp 1.0 65.8
trnR-UCU-atpA_p5 TCGGAATGAAAAGCGTCCAT GGAACGCTTTCTACTTCAAGAAC 45.0 43.5 57.9 58.0 199-206 bp 1.0 64.0