Hotspot

trnS-GCU-trnG-GCC

LSC · 8050-8887 · 838 bp

Back to Bassia

Pi 0.0305
MarkerSeek score 69.60
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
838
variable_sites
55
pi
0.0305
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 ACGGAAAGAGAGGGATTCGA GTAATCAAACCGGGAGAGACC 50.0 52.4 58.2 58.1 1007-1101 bp 1.0 75.2
trnS-GCU-trnG-GCC_p2 CGAATGACTCGTACAACGGA GTAATCAAACCGGGAGAGACC 50.0 52.4 57.7 58.1 977-1071 bp 1.0 74.5
trnS-GCU-trnG-GCC_p3 TGGAAACGGAAAGAGAGGGA GTAATCAAACCGGGAGAGACC 50.0 52.4 58.3 58.1 1012-1106 bp 1.0 74.1
trnS-GCU-trnG-GCC_p4 ACGGAAAGAGAGGGATTCGA TAATCAAACCGGGAGAGACC 50.0 50.0 58.2 56.6 1006-1100 bp 1.0 73.0
trnS-GCU-trnG-GCC_p5 CGAATGACTCGTACAACGGA TAATCAAACCGGGAGAGACC 50.0 50.0 57.7 56.6 976-1070 bp 1.0 72.4