Hotspot

ycf1

SSC · 128269-133932 · 5664 bp

Back to Barringtonia

Pi 0.00053

MarkerSeek score 52.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5664
variable_sites: 3
pi: 0.00053
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGTTGATTCAAGAAAAAGTTGT	TGGCTGATAGAACAAGCACA	27.3	45.0	53.0	57.1	281 bp	1.0	40.6
ycf1_p2	TTGTTGATTCAAGAAAAAGTTGT	TGGCTGATAGAACAAGCACA	26.1	45.0	53.8	57.1	282 bp	1.0	40.6
ycf1_p3	TGTTGATTCAAGAAAAAGTTGT	TCTGGCTGATAGAACAAGCAC	27.3	47.6	53.0	58.0	283 bp	1.0	40.6
ycf1_p4	TGTTGATTCAAGAAAAAGTTGT	TGGCTGATAGAACAAGCACAA	27.3	42.9	53.0	57.9	281 bp	1.0	40.6
ycf1_p5	TTGTTGATTCAAGAAAAAGTTGT	TCTGGCTGATAGAACAAGCAC	26.1	47.6	53.8	58.0	284 bp	1.0	40.6