Hotspot

rps2

LSC · 16541-17251 · 711 bp

Back to Barringtonia

Pi 0
MarkerSeek score 26.80
Primer available yes
Label rps2

Feature Metadata

label_name
rps2
feature_type
gene
region
LSC
length_bp
711
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
26.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2_p1 TCTCTTGTTTGAGGTTCACA GGGGGAGTGTGGGGAAAA 40.0 61.1 54.4 58.7 816 bp 1.0 40.0
rps2_p2 TCTCTTGTTTGAGGTTCACAA GGGGGAGTGTGGGGAAAA 38.1 61.1 55.3 58.7 816 bp 1.0 40.0
rps2_p3 ATCTCTTGTTTGAGGTTCACA GGGGGAGTGTGGGGAAAA 38.1 61.1 55.1 58.7 817 bp 1.0 40.0
rps2_p4 TCTCTTGTTTGAGGTTCACAAA GGGGGAGTGTGGGGAAAA 36.4 61.1 56.1 58.7 816 bp 1.0 40.0
rps2_p5 ACCAATTCCAAAAATCTAGGGAGT GGGGGAGTGTGGGGAAAA 37.5 61.1 58.1 58.7 789 bp 1.0 40.0