Hotspot

rpoC2

LSC · 17477-21634 · 4158 bp

Back to Barringtonia

Pi 0.000241

MarkerSeek score 53.90

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4158
variable_sites: 1
pi: 0.000241
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	AACAAGTCTGCGCGTAAGAT	GGGAGCTAGAGGAAATGCAT	45.0	50.0	57.9	57.1	220 bp	1.0	72.1
rpoC2_p2	AACAAGTCTGCGCGTAAGAT	TTAATGTCGGATCCCCAAGG	45.0	50.0	57.9	57.0	168 bp	1.0	71.7
rpoC2_p3	AACAAGTCTGCGCGTAAGAT	GTCTTTTTCGGGAGCTAGAGG	45.0	52.4	57.9	58.1	229 bp	1.0	70.9
rpoC2_p4	GCCACAATCTGTTCGACGTA	GGGAGCTAGAGGAAATGCAT	50.0	50.0	58.3	57.1	262 bp	1.0	70.4
rpoC2_p5	AACAAGTCTGCGCGTAAGAT	TCTTTTTCGGGAGCTAGAGGA	45.0	47.6	57.9	58.2	228 bp	1.0	70.2