Hotspot

clpP

LSC · 73331-75619 · 2289 bp

Back to Barringtonia

Pi 0.000874

MarkerSeek score 57.70

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2289
variable_sites: 2
pi: 0.000874
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GCGCGAAATCCATGCTATTT	ACGTTTCCACATCAAAGTGA	45.0	40.0	57.6	55.5	2365-2366 bp	1.0	57.0
clpP_p2	GCGCGAAATCCATGCTATTT	ACGTTTCCACATCAAAGTGAA	45.0	38.1	57.6	56.3	2365-2366 bp	1.0	55.3
clpP_p3	TGCGCGAAATCCATGCTATT	ACGTTTCCACATCAAAGTGA	45.0	40.0	58.7	55.5	2366-2367 bp	1.0	54.7
clpP_p4	TTGCGCGAAATCCATGCTAT	ACGTTTCCACATCAAAGTGA	45.0	40.0	58.7	55.5	2367-2368 bp	1.0	54.7
clpP_p5	GCGCGAAATCCATGCTATTT	ACGTTTCCACATCAAAGTGAAA	45.0	36.4	57.6	57.0	2365-2366 bp	1.0	53.1