Hotspot

petA-psbJ

LSC · 66017-67145 · 1129 bp

Back to Barringtonia

Pi 0

MarkerSeek score 58.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1129
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGTTCAAGGCCTTTTGCTCT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.9	57.6	1264-1281 bp	1.0	76.6
petA-psbJ_p2	GTGTTCAAGGCCTTTTGCTC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.9	57.6	1265-1282 bp	1.0	76.1
petA-psbJ_p3	CCATTACGTGTTCAAGGCCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1272-1289 bp	1.0	75.8
petA-psbJ_p4	TCCATTACGTGTTCAAGGCC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1273-1290 bp	1.0	75.7
petA-psbJ_p5	TAGCATCGGTTGTTTTGGCA	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.4	57.6	1241-1258 bp	1.0	74.1