Hotspot

trnM-CAU-atpE

LSC · 55547-55773 · 227 bp

Back to Barringtonia

Pi 0.00881
MarkerSeek score 57.60
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
227
variable_sites
2
pi
0.00881
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 ATGCGATAGGTCACGTTTGG CTCTCAGACGAGCTAGGACA 50.0 55.0 58.3 58.0 409 bp 1.0 77.4
trnM-CAU-atpE_p2 TATTGCTTTCATACGGCGGG CTCTCAGACGAGCTAGGACA 50.0 55.0 58.4 58.0 344 bp 1.0 76.9
trnM-CAU-atpE_p3 ATGCGATAGGTCACGTTTGG GGACACGAGTCGAAGCTATC 50.0 55.0 58.3 57.9 394 bp 1.0 76.8
trnM-CAU-atpE_p4 ATGCGATAGGTCACGTTTGG CGATAGGTCTAGTTGGTGCG 50.0 55.0 58.3 57.9 370 bp 1.0 76.7
trnM-CAU-atpE_p5 ATGCGATAGGTCACGTTTGG GCGATAGGTCTAGTTGGTGC 50.0 55.0 58.3 58.1 371 bp 1.0 76.5