Hotspot

rpoB-trnC-GCA

LSC · 27838-28999 · 1162 bp

Back to Barringtonia

Pi 0

MarkerSeek score 58.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1162
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGTCAAGCCCTGATCAATGA	AGATCAGCAGAACCGAATCG	50.0	50.0	58.0	57.8	1372-1373 bp	1.0	78.1
rpoB-trnC-GCA_p2	CGTCAAGCCCTGATCAATGA	AACAAGGATTTGCAGTCCCC	50.0	50.0	58.0	58.4	1302-1303 bp	1.0	76.9
rpoB-trnC-GCA_p3	CGTCAAGCCCTGATCAATGA	ACTGGGGAACAAGGATTTGC	50.0	50.0	58.0	58.4	1309-1310 bp	1.0	76.9
rpoB-trnC-GCA_p4	CGTCAAGCCCTGATCAATGA	GGGGAACAAGGATTTGCAGT	50.0	50.0	58.0	58.4	1306-1307 bp	1.0	76.9
rpoB-trnC-GCA_p5	CGTCAAGCCCTGATCAATGA	TGGGGAACAAGGATTTGCAG	50.0	50.0	58.0	58.4	1307-1308 bp	1.0	76.9