Hotspot

rps2-rpoC2

LSC · 17252-17476 · 225 bp

Back to Barringtonia

Pi 0.00889

MarkerSeek score 57.60

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 225
variable_sites: 2
pi: 0.00889
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CGAGGGTTCCATTTCTTGGT	TCACGATACACCAGAACTTCT	50.0	42.9	57.8	56.4	354 bp	1.0	58.2
rps2-rpoC2_p2	CGAGGGTTCCATTTCTTGGT	TTCACGATACACCAGAACTTCT	50.0	40.9	57.8	57.1	355 bp	1.0	55.9
rps2-rpoC2_p3	GCCATTCGAGGGTTCCATTT	TCACGATACACCAGAACTTCT	50.0	42.9	58.5	56.4	360 bp	1.0	55.6
rps2-rpoC2_p4	CGAGGGTTCCATTTCTTGGT	ACGATACACCAGAACTTCTAGG	50.0	45.5	57.8	57.0	352 bp	1.0	55.0
rps2-rpoC2_p5	TGGTACCATGGCCAAAATGA	TCACGATACACCAGAACTTCT	45.0	42.9	57.4	56.4	338 bp	1.0	54.9