Hotspot

ycf3

LSC · 42495-44450 · 1956 bp

Back to Barnadesia

Pi 0.00308

MarkerSeek score 49.30

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1956
variable_sites: 6
pi: 0.00308
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TGATGGGGCCAACAAACAAT	TCCCGCGAGAAGTAATGAGA	45.0	50.0	58.3	58.2	2082-2092 bp	1.0	76.5
ycf3_p2	TGATGGGGCCAACAAACAAT	GGATCATTTTCCCGCGAGAA	45.0	50.0	58.3	58.3	2091-2101 bp	1.0	75.7
ycf3_p3	TGATGGGGCCAACAAACAAT	ATCATTTTCCCGCGAGAAGT	45.0	45.0	58.3	57.6	2089-2099 bp	1.0	75.2
ycf3_p4	ATTTGACTGATGGGGCCAAC	TCCCGCGAGAAGTAATGAGA	50.0	50.0	58.4	58.2	2089-2099 bp	1.0	75.2
ycf3_p5	ATTTGACTGATGGGGCCAAC	GGATCATTTTCCCGCGAGAA	50.0	50.0	58.4	58.3	2098-2108 bp	1.0	74.4