Hotspot

rps11

LSC · 78442-78852 · 411 bp

Back to Barnadesia

Pi 0

MarkerSeek score 21.10

Primer available yes

Label rps11

Feature Metadata

label_name: rps11
feature_type: gene
region: LSC
length_bp: 411
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 21.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11_p1	ACTGTTACTTTCTCTCGAACCA	AGGGGATCTGTTTTGACATGA	40.9	42.9	57.1	56.9	584 bp	0.5	20.0
rps11_p2	TGTAGTGTCCGAGTAGATACTGT	AGGGGATCTGTTTTGACATGA	43.5	42.9	57.9	56.9	602 bp	0.5	20.0
rps11_p3	ACTGTTACTTTCTCTCGAACCA	AAGGGGATCTGTTTTGACATGA	40.9	40.9	57.1	57.6	585 bp	0.5	20.0
rps11_p4	ACTGTTACTTTCTCTCGAACCA	AGGGGATCTGTTTTGACATGAA	40.9	40.9	57.1	57.6	584 bp	0.5	20.0
rps11_p5	AGTGTCCGAGTAGATACTGT	AGGGGATCTGTTTTGACATGA	45.0	42.9	54.7	56.9	599 bp	0.5	20.0