Hotspot

ndhE

SSC · 116621-116926 · 306 bp

Back to Barnadesia

Pi 0

MarkerSeek score 20.20

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 20.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TCCTCTGTTCTTGGAGCAGA	CGTGGCCTGTACAAGTCTTT	50.0	50.0	58.0	58.1	1129 bp	1.0	79.3
ndhE_p2	ATATTTTGGGGCACGGGTTT	CGTGGCCTGTACAAGTCTTT	45.0	50.0	58.1	58.1	1304 bp	1.0	79.0
ndhE_p3	ATGATCATACCTTACGCGGC	CGTGGCCTGTACAAGTCTTT	50.0	50.0	57.9	58.1	823 bp	1.0	78.9
ndhE_p4	ACATCCGTCCCAAGGTATCA	CGTGGCCTGTACAAGTCTTT	50.0	50.0	58.1	58.1	1091 bp	1.0	78.4
ndhE_p5	TCCTCTGTTCTTGGAGCAGA	AGCTATCGCATGCATTTCCT	50.0	45.0	58.0	57.7	862 bp	1.0	78.1