Hotspot

ndhE-ndhG

SSC · 116927-117143 · 217 bp

Back to Barnadesia

Pi 0.0184

MarkerSeek score 63.10

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 217
variable_sites: 4
pi: 0.0184
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	GACACATAAGAGCCCTAACCA	TAATCGGCGCAATTGCTGTA	47.6	45.0	57.1	58.3	349 bp	1.0	62.9
ndhE-ndhG_p2	ACAAGTACATGTTCGAGCATCA	TAATCGGCGCAATTGCTGTA	40.9	45.0	58.1	58.3	272 bp	1.0	61.8
ndhE-ndhG_p3	AGTTCAAGACACATAAGAGCCC	TAATCGGCGCAATTGCTGTA	45.5	45.0	58.1	58.3	356 bp	1.0	61.7
ndhE-ndhG_p4	GTTCAAGACACATAAGAGCCCT	TAATCGGCGCAATTGCTGTA	45.5	45.0	58.1	58.3	355 bp	1.0	61.7
ndhE-ndhG_p5	TCAAGACACATAAGAGCCCT	TAATCGGCGCAATTGCTGTA	45.0	45.0	55.9	58.3	353 bp	1.0	61.0