Hotspot

rpl32-trnL-UAG

SSC · 112233-113118 · 886 bp

Back to Barnadesia

Pi 0.00452
MarkerSeek score 61.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
886
variable_sites
4
pi
0.00452
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TATTTGGCGGCGTTAAAAGC AGCGTGTCTACCGATTTCAC 45.0 50.0 57.7 58.0 1008 bp 1.0 78.1
rpl32-trnL-UAG_p2 TATTTGGCGGCGTTAAAAGC CAGCGTGTCTACCGATTTCA 45.0 50.0 57.7 58.0 1009 bp 1.0 78.1
rpl32-trnL-UAG_p3 AGGAAAGGATATTTGGCGGC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.3 58.0 1017 bp 1.0 77.8
rpl32-trnL-UAG_p4 AGGAAAGGATATTTGGCGGC CAGCGTGTCTACCGATTTCA 50.0 50.0 58.3 58.0 1018 bp 1.0 77.8
rpl32-trnL-UAG_p5 AGGATATTTGGCGGCGTTAA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.7 58.0 1012 bp 1.0 77.6