Hotspot

ycf1-ndhF

SSC · 108705-108749 · 45 bp

Back to Barnadesia

Pi 0.0444

MarkerSeek score 78.10

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 45
variable_sites: 2
pi: 0.0444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	TGTTTCGATGCAACAGCAAG	TAGGAGGTGGGCGTATTTCT	45.0	50.0	57.6	57.8	183-187 bp	1.0	76.3
ycf1-ndhF_p2	TGTTTCGATGCAACAGCAAG	GTAGGAGGTGGGCGTATTTC	45.0	55.0	57.6	57.8	184-188 bp	1.0	75.7
ycf1-ndhF_p3	TGTTTCGATGCAACAGCAAG	GGAGGTGGGCGTATTTCTTC	45.0	55.0	57.6	58.3	181-185 bp	1.0	75.0
ycf1-ndhF_p4	GTTTCGATGCAACAGCAAGA	TAGGAGGTGGGCGTATTTCT	45.0	50.0	57.3	57.8	182-186 bp	1.0	74.0
ycf1-ndhF_p5	GTTTCGATGCAACAGCAAGA	GTAGGAGGTGGGCGTATTTC	45.0	55.0	57.3	57.8	183-187 bp	1.0	73.5