Hotspot

rps19-rpl2

IRb · 83603-83661 · 59 bp

Back to Barnadesia

Pi 0.0172

MarkerSeek score 62.20

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 59
variable_sites: 1
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	GCCCGGGACCAGGTTATTAT	GGGCCCCAATTGGTAGAAAA	55.0	50.0	59.2	58.1	287-288 bp	1.0	70.3
rps19-rpl2_p2	GCCCGGGACCAGGTTATTAT	TTGATTCTTCGTCGCCGTAG	55.0	50.0	59.2	58.1	195-196 bp	1.0	70.2
rps19-rpl2_p3	GCCCGGGACCAGGTTATTAT	CACAACCCCTTGGGGTTATC	55.0	55.0	59.2	58.2	262-263 bp	1.0	69.5
rps19-rpl2_p4	CCCGGGACCAGGTTATTATT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	56.7	58.1	286-287 bp	1.0	69.4
rps19-rpl2_p5	CCCGGGACCAGGTTATTATT	TTGATTCTTCGTCGCCGTAG	50.0	50.0	56.7	58.1	194-195 bp	1.0	69.3