Hotspot

rps8-rpl14

LSC · 79930-80101 · 172 bp

Back to Barnadesia

Pi 0.0233

MarkerSeek score 65.90

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 172
variable_sites: 4
pi: 0.0233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGTAGATGCTACCCGAACCA	GAACTCGAGTTTTTGGTGCG	50.0	50.0	57.8	58.0	345-351 bp	1.0	79.5
rps8-rpl14_p2	AGTAGATGCTACCCGAACCA	CGATTGCCCGAGAATTGAGA	50.0	50.0	57.8	58.1	327-333 bp	1.0	79.3
rps8-rpl14_p3	GTAGATGCTACCCGAACCAC	GAACTCGAGTTTTTGGTGCG	55.0	50.0	57.8	58.0	344-350 bp	1.0	79.0
rps8-rpl14_p4	AGTAGATGCTACCCGAACCA	TTGCCCGAGAATTGAGACAG	50.0	50.0	57.8	57.9	324-330 bp	1.0	78.9
rps8-rpl14_p5	AGTAGATGCTACCCGAACCA	GCCCGAGAATTGAGACAGTT	50.0	50.0	57.8	57.9	322-328 bp	1.0	78.9