Hotspot

psaA-ycf3

LSC · 41779-42494 · 716 bp

Back to Barnadesia

Pi 0.00559

MarkerSeek score 62.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 4
pi: 0.00559
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GAGAAATGACCCGGTCTAGC	AGCCGCTGAGTATTGGAAAC	55.0	50.0	57.8	58.3	908-909 bp	1.0	76.5
psaA-ycf3_p2	GAGAAATGACCCGGTCTAGC	CAAGCCGCTGAGTATTGGAA	55.0	50.0	57.8	58.3	910-911 bp	1.0	76.4
psaA-ycf3_p3	GAGAAATGACCCGGTCTAGC	TGGTTGAAGATCACGAGACG	55.0	50.0	57.8	57.7	844-845 bp	1.0	75.8
psaA-ycf3_p4	GAGAAATGACCCGGTCTAGC	GGTTGAAGATCACGAGACGT	55.0	50.0	57.8	57.7	843-844 bp	1.0	75.8
psaA-ycf3_p5	GAGAAATGACCCGGTCTAGC	AAGCCGCTGAGTATTGGAAA	55.0	45.0	57.8	57.5	909-910 bp	1.0	74.6