Hotspot

trnE-UUC-trnT-GGU

LSC · 30925-31527 · 603 bp

Back to Barnadesia

Pi 0
MarkerSeek score 61.40
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
603
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 GAAGTCCTAACCGCTAGACG GAACCGATGACTTACGCCTT 55.0 50.0 57.5 58.0 620-686 bp 1.0 80.2
trnE-UUC-trnT-GGU_p2 AGAGAGAAGTCCTAACCGCT GAACCGATGACTTACGCCTT 50.0 50.0 57.5 58.0 625-691 bp 1.0 79.8
trnE-UUC-trnT-GGU_p3 GAAGTCCTAACCGCTAGACG CCATGGCGTTACTCTACCAC 55.0 55.0 57.5 58.1 599-665 bp 1.0 79.7
trnE-UUC-trnT-GGU_p4 CCCGTTGTCTCCTTGAAAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.5 58.0 642-708 bp 1.0 79.6
trnE-UUC-trnT-GGU_p5 TCCCGTTGTCTCCTTGAAAG GAACCGATGACTTACGCCTT 50.0 50.0 57.5 58.0 643-709 bp 1.0 79.6