Hotspot

trnC-GCA-petN

LSC · 28385-29208 · 824 bp

Back to Barnadesia

Pi 0.00147

MarkerSeek score 60.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 824
variable_sites: 1
pi: 0.00147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TCTCCTATCATTTTGGCGGC	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.0	58.2	799-936 bp	1.0	82.6
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	743-880 bp	1.0	82.2
trnC-GCA-petN_p3	CTCCTATCATTTTGGCGGCA	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.3	58.2	798-935 bp	1.0	80.3
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	761-898 bp	1.0	80.0
trnC-GCA-petN_p5	TCCTATCATTTTGGCGGCAT	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.6	58.2	797-934 bp	1.0	80.0