Hotspot

atpH-atpI

LSC · 13817-14798 · 982 bp

Back to Barnadesia

Pi 0

MarkerSeek score 63.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 982
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	GTGAATCCATGGAGGGTCAC	45.0	55.0	58.0	58.0	986-1040 bp	1.0	83.3
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	GTGAATCCATGGAGGGTCAC	50.0	55.0	57.9	58.0	1042-1096 bp	1.0	82.7
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	GTGAATCCATGGAGGGTCAC	45.0	55.0	57.9	58.0	1002-1056 bp	1.0	82.5
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	GTGAATCCATGGAGGGTCAC	45.0	55.0	58.2	58.0	987-1041 bp	1.0	82.2
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	GTGAATCCATGGAGGGTCAC	55.0	55.0	58.2	58.0	1046-1100 bp	1.0	82.1