Hotspot

rps16-trnQ-UUG

LSC · 6235-7169 · 935 bp

Back to Barnadesia

Pi 0.00759
MarkerSeek score 64.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
935
variable_sites
7
pi
0.00759
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 997-1008 bp 1.0 79.8
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1008-1019 bp 1.0 79.3
rps16-trnQ-UUG_p3 CACACCGGATCATGTCCTTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.3 58.1 1017-1028 bp 1.0 78.1
rps16-trnQ-UUG_p4 CACCGGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 1015-1026 bp 1.0 77.7
rps16-trnQ-UUG_p5 CCGGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.3 58.1 1013-1024 bp 1.0 75.3