Hotspot

ndhA

SSC · 117951-120116 · 2166 bp

Back to Barleria

Pi 0.0206

MarkerSeek score 57.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2166
variable_sites: 84
pi: 0.0206
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CGTGGGACAGGGTAATCATG	GCCGGGTTTTATCAACTTGC	55.0	50.0	58.0	58.0	2452-2465 bp	1.0	82.8
ndhA_p2	CGTGGGACAGGGTAATCATG	CAATGTTACGAGCTTCCGGA	55.0	50.0	58.0	58.0	2855-2868 bp	1.0	82.4
ndhA_p3	ATTCTCGCCAACCAACCATT	GCCGGGTTTTATCAACTTGC	45.0	50.0	58.1	58.0	2965-2978 bp	1.0	82.3
ndhA_p4	CGTGGGACAGGGTAATCATG	CGAGTTGAAGGCATAGGCAT	55.0	50.0	58.0	58.0	2916-2929 bp	1.0	82.1
ndhA_p5	CGTGGGACAGGGTAATCATG	CCGGGGTTGCTGAATATCAA	55.0	50.0	58.0	57.9	2969-2982 bp	1.0	82.0