Hotspot

ndhF-rpl32

SSC · 111165-111579 · 415 bp

Back to Barleria

Pi 0.0345

MarkerSeek score 67.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 415
variable_sites: 26
pi: 0.0345
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CAAGAGGTCGTGTGAACCAA	TTAACGCTGCCCAATATCCC	50.0	50.0	58.1	58.0	1014-1089 bp	1.0	83.2
ndhF-rpl32_p2	AAACCAAAGTCCCCTACACG	TTAACGCTGCCCAATATCCC	50.0	50.0	57.7	58.0	1069-1144 bp	1.0	81.3
ndhF-rpl32_p3	ACCAAAGTCCCCTACACGAT	TTAACGCTGCCCAATATCCC	50.0	50.0	58.3	58.0	1067-1142 bp	1.0	80.7
ndhF-rpl32_p4	ACATGAAGCGGAAATTGTGC	TTAACGCTGCCCAATATCCC	45.0	50.0	57.6	58.0	1246-1321 bp	1.0	80.4
ndhF-rpl32_p5	CAAGAGGTCGTGTGAACCAA	GCTGCCCAATATCCCTTTCT	50.0	50.0	58.1	57.6	1009-1084 bp	1.0	80.4