Hotspot

rps19-rpl2

IRb · 83632-83691 · 60 bp

Back to Barleria

Pi 0.0544

MarkerSeek score 70.30

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 60
variable_sites: 5
pi: 0.0544
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	AGATGAAGGGCTACAAAAGGA	GATTGTTCGTCGCCGTAGTA	42.9	50.0	56.6	57.8	121-132 bp	1.0	66.7
rps19-rpl2_p2	AGATGAAGGGCTACAAAAGGA	TTTGATTGTTCGTCGCCGTA	42.9	45.0	56.6	58.2	124-135 bp	1.0	66.3
rps19-rpl2_p3	AGATGAAGGGCTACAAAAGGA	AATTTGATTGTTCGTCGCCG	42.9	45.0	56.6	57.8	126-137 bp	1.0	66.1
rps19-rpl2_p4	AGATGAAGGGCTACAAAAGGA	TTGTTCGTCGCCGTAGTAAA	42.9	45.0	56.6	57.6	119-130 bp	1.0	64.9
rps19-rpl2_p5	AGATGAAGGGCTACAAAAGGA	TGTTCGTCGCCGTAGTAAAT	42.9	45.0	56.6	57.4	118-129 bp	1.0	63.1