Hotspot

ndhC-trnV-UAC

LSC · 49901-50990 · 1090 bp

Back to Barleria

Pi 0.0166

MarkerSeek score 66.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1090
variable_sites: 34
pi: 0.0166
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAATCCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1159-1242 bp	1.0	84.0
ndhC-trnV-UAC_p2	GGGGCTAAAATCCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1158-1241 bp	1.0	81.3
ndhC-trnV-UAC_p3	GGGGCTAAAATCCCGGAAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1147-1230 bp	1.0	80.1
ndhC-trnV-UAC_p4	GGGGCTAAAATCCCGGAAAT	AAGGTCTACGGTTCGAGTCC	50.0	55.0	57.9	58.8	1157-1240 bp	1.0	79.0
ndhC-trnV-UAC_p5	GGGGCTAAAATCCCGGAAAT	GAGAAGGTCTACGGTTCGAG	50.0	55.0	57.9	57.2	1160-1243 bp	1.0	79.0