Hotspot

psbZ-trnG-GCC

LSC · 35767-35981 · 215 bp

Back to Barleria

Pi 0.0326

MarkerSeek score 71.90

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 215
variable_sites: 14
pi: 0.0326
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CATTTGCTTCTCCTGGTGGT	AATCGAACCCGCATCTTCTC	50.0	50.0	58.1	58.1	382-387 bp	1.0	84.9
psbZ-trnG-GCC_p2	CATTTGCTTCTCCTGGTGGT	GAATCGAACCCGCATCTTCT	50.0	50.0	58.1	58.1	383-388 bp	1.0	84.9
psbZ-trnG-GCC_p3	GTTGTATTTTCCGGCGCATC	AATCGAACCCGCATCTTCTC	50.0	50.0	58.2	58.1	344-349 bp	1.0	84.8
psbZ-trnG-GCC_p4	GTTGTATTTTCCGGCGCATC	GAATCGAACCCGCATCTTCT	50.0	50.0	58.2	58.1	345-350 bp	1.0	84.8
psbZ-trnG-GCC_p5	TCCTGGTGGTTGGTCAAGTA	AATCGAACCCGCATCTTCTC	50.0	50.0	58.2	58.1	372-377 bp	1.0	84.2