Hotspot

petN-psbM

LSC · 28515-29503 · 989 bp

Back to Barleria

Pi 0.0227

MarkerSeek score 66.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 989
variable_sites: 44
pi: 0.0227
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	TCTAGTTCCTACCGCCTTTCT	50.0	47.6	58.0	57.9	1064-1081 bp	1.0	76.9
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	TCTAGTTCCTACCGCCTTTCT	50.0	47.6	58.0	57.9	1062-1079 bp	1.0	76.9
petN-psbM_p3	CGTAGTGTGGGGAAGAAGTG	TCTAGTTCCTACCGCCTTTCT	55.0	47.6	57.9	57.9	1061-1078 bp	1.0	76.3
petN-psbM_p4	ACTCGTAGTGTGGGGAAGAA	AGTTCCTACCGCCTTTCTACT	50.0	47.6	58.0	58.2	1061-1078 bp	1.0	76.2
petN-psbM_p5	TCGTAGTGTGGGGAAGAAGT	AGTTCCTACCGCCTTTCTACT	50.0	47.6	58.0	58.2	1059-1076 bp	1.0	76.2