Hotspot

atpH-atpI

LSC · 13080-14090 · 1011 bp

Back to Barleria

Pi 0.0164

MarkerSeek score 66.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1011
variable_sites: 34
pi: 0.0164
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.1	57.9	1090-1156 bp	1.0	81.0
atpH-atpI_p2	GCCAATCCAGCAGCAATAAC	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.8	57.9	1107-1173 bp	1.0	80.1
atpH-atpI_p3	TCCAGCAGCAATAACGGAAG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.3	57.9	1102-1168 bp	1.0	79.7
atpH-atpI_p4	AATCCAGCAGCAATAACGGA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.6	57.9	1104-1170 bp	1.0	78.5
atpH-atpI_p5	ATCCAGCAGCAATAACGGAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.6	57.9	1103-1169 bp	1.0	78.5