Hotspot

trnH-GUG-psbA

LSC · 79-521 · 443 bp

Back to Barleria

Pi 0.0381
MarkerSeek score 78.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
443
variable_sites
34
pi
0.0381
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CGTGCTAACCTTGGTATGGA 55.0 50.0 58.1 57.3 557-644 bp 1.0 81.4
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TCGTGCTAACCTTGGTATGG 55.0 50.0 58.1 57.3 558-645 bp 1.0 81.3
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA CGTGCTAACCTTGGTATGGA 50.0 50.0 57.9 57.3 571-658 bp 1.0 81.1
trnH-GUG-psbA_p4 CAATCCACTGCCTTGATCCA TCGTGCTAACCTTGGTATGG 50.0 50.0 57.9 57.3 572-659 bp 1.0 81.1
trnH-GUG-psbA_p5 AATCCACTGCCTTGATCCAC CGTGCTAACCTTGGTATGGA 50.0 50.0 57.9 57.3 570-657 bp 1.0 81.1