Hotspot

atpF

LSC · 56552-57940 · 1389 bp

Back to Barfussia

Pi 0.0036

MarkerSeek score 51.10

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1389
variable_sites: 5
pi: 0.0036
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACTAATTTCGTCGGCTCGAA	GAAAGGGGCGAGTGAAGTAA	45.0	50.0	57.4	57.5	1567-1568 bp	1.0	71.4
atpF_p2	GGCTCGAAGGGTTACCATTA	GAAAGGGGCGAGTGAAGTAA	50.0	50.0	57.0	57.5	1555-1556 bp	1.0	68.6
atpF_p3	TCTTTTTCGGAAGCAAAGGGA	GAAAGGGGCGAGTGAAGTAA	42.9	50.0	58.1	57.5	1523-1524 bp	1.0	68.1
atpF_p4	GGCTCGAAGGGTTACCATTAG	GAAAGGGGCGAGTGAAGTAA	52.4	50.0	58.2	57.5	1555-1556 bp	1.0	67.2
atpF_p5	ACTAATTTCGTCGGCTCGAA	GAAAGGGGCGAGTGAAGTAAT	45.0	47.6	57.4	58.0	1567-1568 bp	1.0	67.1