Hotspot

ycf2-ycf15

IRa · 140248-140315 · 68 bp

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Pi 0.0147

MarkerSeek score 61.00

Primer available yes

Label ycf2-ycf15

Feature Metadata

label_name: ycf2-ycf15
feature_type: igs
region: IRa
length_bp: 68
variable_sites: 1
pi: 0.0147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-ycf15_p1	AAAGAGATGGCTTTTCCCGG	TCCATGTTTCAGCAGTAGCA	50.0	45.0	58.2	57.1	214 bp	1.0	72.2
ycf2-ycf15_p2	ATGGCTTTTCCCGGATGAAA	TCCATGTTTCAGCAGTAGCA	45.0	45.0	57.8	57.1	208 bp	1.0	71.7
ycf2-ycf15_p3	TGGCTTTTCCCGGATGAAAT	TCCATGTTTCAGCAGTAGCA	45.0	45.0	57.8	57.1	207 bp	1.0	71.7
ycf2-ycf15_p4	GATGGCTTTTCCCGGATGAA	TCCATGTTTCAGCAGTAGCA	50.0	45.0	58.2	57.1	209 bp	1.0	71.6
ycf2-ycf15_p5	AAAGAGATGGCTTTTCCCGG	TGTTCCATGGAGCTAAGGTC	50.0	50.0	58.2	56.9	191 bp	1.0	70.5