Hotspot

petA-psbJ

LSC · 108981-110096 · 1116 bp

Back to Barfussia

Pi 0.00358
MarkerSeek score 60.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1116
variable_sites
4
pi
0.00358
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCCTTTTGTTCTTCTTCGC TCTGGGTTGGGTTCATCTCT 50.0 50.0 57.9 58.0 1242-1265 bp 1.0 79.5
petA-psbJ_p2 GGCCTTTTGTTCTTCTTCGC TGGGTTGGGTTCATCTCTCT 50.0 50.0 57.9 58.0 1240-1263 bp 1.0 79.5
petA-psbJ_p3 GGCCTTTTGTTCTTCTTCGC CTGGGTTGGGTTCATCTCTC 50.0 55.0 57.9 57.6 1241-1264 bp 1.0 76.6
petA-psbJ_p4 CGTCCAAGGCCTTTTGTTCT TCTGGGTTGGGTTCATCTCT 50.0 50.0 58.7 58.0 1249-1272 bp 1.0 74.4
petA-psbJ_p5 CGTCCAAGGCCTTTTGTTCT TGGGTTGGGTTCATCTCTCT 50.0 50.0 58.7 58.0 1247-1270 bp 1.0 74.4