Hotspot

ycf4-cemA

LSC · 106247-107097 · 851 bp

Back to Barfussia

Pi 0.00235

MarkerSeek score 60.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 851
variable_sites: 2
pi: 0.00235
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	ACAAAAAGCTGCCGAATTGG	TGGGAAAGAAGGCAATGCTT	45.0	45.0	57.8	58.0	939 bp	1.0	78.8
ycf4-cemA_p2	ACAAAAAGCTGCCGAATTGG	ATGAGAGAGAGACCCACCAG	45.0	55.0	57.8	57.8	985 bp	1.0	77.7
ycf4-cemA_p3	GCCATTCCCTTGACTCGTAC	TGGGAAAGAAGGCAATGCTT	55.0	45.0	58.3	58.0	989 bp	1.0	77.3
ycf4-cemA_p4	ACAAAAAGCTGCCGAATTGG	ATGGGAAAGAAGGCAATGCT	45.0	45.0	57.8	57.8	940 bp	1.0	77.0
ycf4-cemA_p5	GCCATTCCCTTGACTCGTAC	ATGAGAGAGAGACCCACCAG	55.0	55.0	58.3	57.8	1035 bp	1.0	76.1